The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 1 OD at 230 nm. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. 96-well-plate-scan-time-2-colors-1-site-well. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. 04 sec. 98 Bovine serum albumin mg/mL O. Insert the filled microplate into the drawer, matching well A1 with position A1. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. SpectraMax iD5. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. StakMax. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Technical specifications. 8. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). flexibility of monochromator-based systems. Multimode Reader Comparison Chart. Reactions catalyzed by firefly and *Renilla luciferases. Molecular Devices provides. Description. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. microplates or in a cuvette at a. Fluorescence Microplate Reader Comparison Chart. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Microplate Reader (Molecular Devices cat. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. a white 96-well half-area microplate (Corning cat. Published Dec 16, 2020 | Updated Dec 16, 2020. 54: 1. For more sample throughput, the system can be. 96, 384, 24, 12, 6 well types. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. 2. It measures the optical density (OD) of samples in both 96- and 384-well. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 96-well: 0. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. M3-M5e_manual. The CellTiter-Glo assay from Promega uses luciferase enzyme. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. ©2010 molecular Devices, inc. Determine DNA concentrations precisely and . It also integrates with the powerful SoftMax Pro Software for. 01. Use the provided 3. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. • 0. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. Step 2: Click the Read button in SoftMax Pro Software. Documentation:1. Or scan up to 384 wells at any time to determine the best assay conditions. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. This unlocks the transport slide. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Molecular Devices' microplate readers. book Page 9 Wednesday, July 7, 2010 2:09 PM. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. g. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. M5. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Transfer of an assay to 96-well microplate technology increases throughput significantly. No filter change with dual monochromator optics. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Reading a Microplate . Step 3: Click “Settings” and a screen will appear (Figure 1). All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. A. When reading at wavelengths. Or scan up to 384 wells at any time to determine the best assay conditions. Cells were loaded with 2 µM Fluo-4 for one hour. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . style. The OptiMax by Molecular Devices is a tunable microplate reader. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. . Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. ValitaTiter Plus 96-well (1 plate) VAL004. #G7294). (FP) configured microplate readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 48 W x 12. Optimized for fluorescence microplate readers. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Go to Product Details. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 5 mm and 15. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. When reading at wavelengths. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. The Molecular Devices Versamax Microplate Reader INCLUDED:. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Figure 2-23: Bottle holder To install the bottle holder: 1. Flexible for 6-well to 384-well microplates and cuvettes. Setup Guide. A single-mode plate reader is a good starting point. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. to each well. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Bioz Stars score: 86/100, based on 1 PubMed citations. in space. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Materials. Fluorescence Microplate Reader Comparison Chart. Measure more accurately. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. 384 well polystyrene microplates have plate dimensions of 127. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. 96-well microplate at 200 µL/well. #N207) LAL Reagent Water (Lonza cat. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Page: 1/4. 0. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Description. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. This reader's dual monochromators for. iD5. 300 µL of Detergent Compatible Bradford reagent was added to each well. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. A control well was set up to verify. 384 Awareness Technology ChroMate BioTek Synergy Mx. #0310-5227) 96-well cell wash head (Molecular Devices Cat. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Molecular Devices gmbH Tel. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. Flexibility to read a variety of plate formats. Avoid losing high signal. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. 39. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. the appropriate well of the microplate. Paradigm: Delta mP. It features a modular design that allows users to add or change detection cartridges according to their needs. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. #N207) LAL Reagent Water (Lonza cat. Efficient cell washing with minimal cell loss and optimal assay performance. 4 Pages. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Protein quantitation Cross-talk < 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Detergent Compatible Bradford assay. The system provides options for 20-, 40-,. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. 2 2600A ValitaTiter. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 0 96 Well Plate. Microplate Formats: 6, 12, 24, 48, 96, 384. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 5. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. 1. High. 19 H mm that meet microplate industry standards. explore more. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. Condition: Used - Good. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Compare this item. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. The EMax Plus reader measures flat and round 96-well microplates. 69: 85. Softmax Pro GxP Software. the iD5 reader’s filter system. A preconfigured. iD5. Molecular Devices has provided scientists with . Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). SpectraMax® 340PC384 Microplate Reader for. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. 96-well microplate; Immunoassay analyzer;VersaMax price. # 0310-5218T). D. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Products : SpectraMax M2/M2e reader, SpectraMax. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. #PLUS 384) Methods Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. #3676). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Standard SBS 96 and 384 well plates (128. /well integration in 96-well luminescence mode and 0. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. 100 μL/well Steady-Luc working solution was added to the wells. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Shaking: Linear and Orbital. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 2 2601A ValitaTiter. ScanLater Western Blot Detection System. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. When the user applies a set temperature of 37°C, the top is heated to 37. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. of 0. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. The SpectraMax ABS. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. measurements in compatible Molecular Devices® microplate readers. 1-color 96-well . Other features and benefits include: Eliminate changing out filters. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). emax and Vmax microplate readers. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Unparalleled performance on a personalized platform. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. Growth medium was then replaced with fatty acid uptake solution. 92: 88. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. 5% at 1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 2. 7°C and the bottom to 36. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 1536-well: 0. A microplate reader as . Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. Please refer to the Obsolete Microplate Reader Models for more information. Step 2: Launch SoftMax Pro Software. 125 pM * Specifications listed are for europium 96-well: 0. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. 2. Industry standard, 96-well plates are. Please refer to the Obsolete Microplate Reader Models for more information. BSA concentration (µg/mL) Average OD. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 0 OD Photometric resolution: 0. Dynamic range > 9 orders of magnitude. Abstract and Figures. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. Add 150 µL reagent to each well. Published Dec 16, 2020 | Updated Dec 06, 2021. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. reagent basin for ease of pipetting into. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. 2 nm Wavelength Bandpass 2. and luminescence microplate reader validation DATA SHEET. 1. 96-well solid bottom, clear polystyrene microplates (Greiner cat. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Find a new or used microplate reader and lab equipment for sale and auction at LabX. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Products : General, FilterMax F5 reader. Outer wells are aligned for proper viewing with FLIPR. Place the microplate in the drawer. or ValitaTiter Plus (Molecular Devices cat. #W50-100) SpectraMax® ABS Plus Microplate Reader. Microplate Readers. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Align the two slots on the bottle holder with the two pegs on the rail. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. Highly selective for dsDNA over ssDNA or RNA. Endpoint, kinetic, spectrum and area-well scanning Click here. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Microplate readers use a vertical light path so the distance of the. Description. Microplate luminescent reader assay for ERK activity. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. HPV Serology Laboratory Standard Operating Procedure . #3610) BrightMax sealing films (Genesee cat. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. The SpectraMax® ABS Plus Microplate Reader can run . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Specifications for the SpectraMax L microplate reader. Pick the best speed, sensitivity, and price for your needs. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm.